Journal: Advanced Science

Article Title: Targeted 2‐Deoxy‐ D ‐Ribose Delivery by Biomimetic Nanoplatform Activates EGFR for Accelerated Heart Valve Endothelialization

doi: 10.1002/advs.202514170

Figure Lengend Snippet: Fabrication and characterization of antibody‐functionalized red blood cell membrane‐camouflaged NPs (Ab‐RBCM)/2‐deoxy ‐D ‐ribose (2dDR)@poly(lactic‐ co‐ glycolic acid) (PLGA). A) Schematic representation of the fabrication process. B) TEM images of NPs at different fabrication stages. (Scale bars: 200 nm). C) Dynamic Light Scattering (DLS) size distribution, D) zeta potential measurements, and E) Fourier‐transform infrared spectra of NPs at different fabrication stages. ( n = 3). F) Coomassie blue staining (left) and CD47 Western blot analysis (right) of RBCMs during antibody modification. G) Flow cytometric analysis of biotinylated red blood cells with streptavidin–phycoerythrin and Ab‐FITC. H) 2dDR release profile of Ab‐RBCM/2dDR@PLGA. I) Flow cytometric analysis after co‐culture with the endocardium (upper) and macrophages (lower). J) Confocal fluorescence microscopy images after 24 h of co‐culture with the endocardium and macrophages. (Scale bars: 10 µm). Significance levels are denoted as ** P < 0.01; **** P < 0.0001.

Article Snippet: Human VE‐Cadherin Biotinylated Antibody was obtained from R&D Systems Inc. CCK‐8 Kit was obtained from Dojindo Molecular Technologies.

Techniques: Membrane, Zeta Potential Analyzer, Fourier Transform Infrared Spectroscopy, Staining, Western Blot, Modification, Co-Culture Assay, Fluorescence, Microscopy

Journal: British Journal of Cancer

Article Title: CXCL9 induces chemotaxis, chemorepulsion and endothelial barrier disruption through CXCR3-mediated activation of melanoma cells

doi: 10.1038/sj.bjc.6606056

Figure Lengend Snippet: Expression of CXCR3 on human melanoma cells. ( A ) Human melanoma cells isolated from melanoma metastases were stained for surface CXCR3 and analysed by flow cytometry. Grey and bold histogram, CXCR3 staining; black or spotted histogram, isotype staining. ( B ) Melanoma-7 were immunostained with anti-CXCR3 antibody, Alexa488-conjugated anti-mouse as secondary reagent, and for actin with phalloidin-568 and viewed by confocal laser scanning microscopy; bar represents 50 μ m. ( C ) Melanoma-15 was co-cultured with HUVECs and stained with anti-CXCR3 (Alexa-488) and anti-CD144 (Alexa-633) antibodies; bar represents 50 μ m. ( D ) Immunolocalisation of CXCR3 in human skin and melanoma metastasis. Cryosections of human skin and melanoma metastasis were stained with anti-CXCR3 antibody (Alexa-488), anti-CD144 antibody (Alexa-633) and phalloidin-568.

Article Snippet: FITC-conjugated anti-CXCR3 mAb and biotinylated goat IgG anti-human CD144 were purchased from R&D Systems, PE-conjugated anti-CD34 from BD Biosciences (San Jose, CA, USA) and PC5-conjugated anti-CD144 from Immunotech (Marseille, France).

Techniques: Expressing, Isolation, Staining, Flow Cytometry, Confocal Laser Scanning Microscopy, Cell Culture

Journal: British Journal of Cancer

Article Title: CXCL9 induces chemotaxis, chemorepulsion and endothelial barrier disruption through CXCR3-mediated activation of melanoma cells

doi: 10.1038/sj.bjc.6606056

Figure Lengend Snippet: Expression of CXCR3 ligands, CXCL9 and CXCL10, in human tumour endothelial cells. ( A ) Immunolocalisation of CXCL9 in human skin and melanoma metastases. Cryosections of normal human skin and melanoma metastasis (lymph node) were stained with anti-CXCL9 antibody (Alexa-488), anti-CD144 antibody (Alexa-568), and phalloidin-568 and viewed by confocal laser scanning microscopy; bar represents 50 μ m. Insert indicates tumour vessel in a higher magnification (dotted structure). ( B , C ) Quantitative Real-time PCR of CXCL9 and CXCL10. Blood ECs, LECs and TuECs were isolated from human skin and melanoma metastasis by cell sorting using anti-CD34, anti-CD144 and anti-podoplanin antibodies, total RNA was isolated, reverse transcribed and corresponding cDNA subjected to TaqMan PCR using commercial probes and primers for CXCL9 and CXCL10. All data shown are mean values and standard error of means from two independent PCR measurements, and have been normalised to the internal control gene B2M (N, normal skin; T, malignant melanoma).

Article Snippet: FITC-conjugated anti-CXCR3 mAb and biotinylated goat IgG anti-human CD144 were purchased from R&D Systems, PE-conjugated anti-CD34 from BD Biosciences (San Jose, CA, USA) and PC5-conjugated anti-CD144 from Immunotech (Marseille, France).

Techniques: Expressing, Staining, Confocal Laser Scanning Microscopy, Real-time Polymerase Chain Reaction, Isolation, FACS, Reverse Transcription, Control